cholesterol synthesis is regulated by ..

In order to detect the effect of RBF on the activityof HMG-CoA reductase, this rate-limiting enzyme in cholesterolbiosynthesis was also investigated. As shown in , RBF inhibits HMG-CoA reductaseactivity in a dose-dependent manner and the concentration of RBFrequired for 50% inhibition of enzymatic activity was ~3 mM.

the de novo synthesis of cholesterol by the ..

07/01/2018 · Inhibition of cholesterol synthesis leads to reduced ..

Differences in synthesis and absorption of cholesterol …

To investigate the mechanism of inhibition of RBF oncholesterol synthesis, the effect of RBF on the incorporation intocholesterol of radiolabeled acetate and radiolabeled mevalonate inrat hepatoma cell culture was measured. The results of thisexperiment suggested that the inhibition of cholesterol synthesisappears to be mediated primarily at the level of HMG-CoA reductase,since inhibition of the incorporation of radiolabeled acetate wasmarkedly higher than that of radiolabeled mevalonate, the productof HMG-CoA reductase. This finding is consistent with the study bySingh () on blacktea extracts, which also predominantly consist of a diverse mixtureof polymerized polyphenols. Furthermore, treatment of RBF inmicrosomal preparations was found to directly inactivate HMG-CoAreductase, indicating that RBF may suppress cholesterol synthesisvia inactivation of HMG-CoA reductase, the rate-controlling enzymein cholesterol biosynthesis (,).

Cholesterol synthesis by human fetal hepatocytes: …

The present study evaluated the cholesterol-loweringeffect of RBF in rats. RBF treatment significantly decreased serumTC, indicating a potential role for RBF in the treatment ofatherosclerosis. This effect was replicated in rat hepatoma cells,in which a 72% decrease of cholesterol synthesis at 100 μg/ml RBFwas observed. These results suggest that inhibition of cholesterolsynthesis may contribute to the effect of RBF on HMG-CoA and .

Espenshade PJ and Hughes AL: Regulation ofsterol synthesis in eukaryotes. Annu Rev Genet. 41:401–427. 2007. :  :

The straight dope on cholesterol – Part VI

The precise mechanisms are unclear, but evidence indicates that other factors including diet, body fat, weight loss, and hormone and enzyme activity interact with exercise to alter the rates of synthesis, transport and clearance of cholesterol from the blood (Durstine & Haskell 1994).

Taking a statin reduces your synthesis of both cholesterol and co-enzyme Q10 which may ..

4-FA-Regulation-Cholesterol-Synthesis-2016-4 - Acetyl …

A number of studies have demonstrated thatflavonoids, together with other polyphenols, such as luteolin andquercetin, decreased cholesterol synthesis by direct inhibition ofsqualene monooxygenase, a rate-limiting enzyme involved incholesterol synthesis (–). These results have suggested thatpolyphenols may inhibit 3-hydroxy-3-methylglutaryl-coenzyme A(HMG-CoA) reductase, an important enzyme in the synthesis ofcholesterol (,–).However, RBF is an oligomeric condensed polyphenol, which may exertadditive effects, compared with those of individual polyphenols, oncholesterol synthesis. In view of its directly and indirectlyinfluencing cholesterol synthesis, together with its nature andsafety characteristics, RBF may be useful as a therapeutic compoundfor the prevention of atherosclerosis. However, the majority ofstudies to date have been conducted , which issignificantly more simplistic than the process. Inaddition, the mechanisms by which RBF reduces cholesterol have notyet been established.

Human trials then confirmed the fluctuation in cholesterol synthesis, ..

Cholesterol | C27H46O | ChemSpider

Robinetinidol-(4β,2′)-tetrahydroxy-flavone (RBF,), is found in the bark ofthe leguminous tree De Wild and has beenshown to exhibit anti-obesity properties (). The mechanism by which RBF affectsobesity may be attributable to its effect on cholesterol synthesis(), since cholesterol is crucialto the development of atherosclerosis (). RBF may therefore prove useful in thetreatment of atherosclerosis.

That’s because cholesterol synthesis is up-regulated as a result of the body ..

High-density lipoprotein - Wikipedia

According to FNRI’s research, camote and other root crops can prevent the re-absorption of bile acids in the liver. Bile acids are converted to cholesterol in the liver and goes to the arteries. Based on their study, the dietary fiber content of root crops has 4.6 to13.5 grams per 100 grams. FNRI explained that root crops are not digested in the small intestine, but fermented in the colon by friendly bacteria. When it is fermented, these can produce short chain fatty acids (SCFA) which can help prevent cholesterol synthesis.