Obesity Is Associated with Increased Red Blood Cell Folate …
11 Red Blood Cell Disorders | Anemia | Folic Acid
There are a number of methods which can be utilized to quantitate serum or red cell folate. These include microbiologic assays, competitive protein-binding assays, or chromatography. A majority of laboratories, including Mayo, use a protein-binding assay with chemiluminescent detection for both serum and red blood cell folate, where the red cell hemolysate is prepared by lysing with ascorbic acid, which releases the intracellular folate, again primarily in the 5-tetrahydrofolate form.
Formation of Red Blood Cells (Erythrocytes) Folate ..
Analytical imprecision is notably worse with red cell folate assays compared to serum folate assays. This has been well documented in the literature and noticeable in proficiency testing surveys, where the variability even within the same peer group may be up to 50%. Manual sample preparation and variability among procedures to completely lyse the red blood cells are likely responsible for much of this variation.