Synpeptide Co.,Ltd.-Custom Peptide synthesis
Structural Biochemistry/Proteins/Synthesis - …
Repeated incomplete deprotection of the αamino function as well as difficulties in obtaining a complete coupling reaction are some of the problems caused by the on resin aggregation of the peptide chain.
amino acids in length is automated solid-phase peptide synthesis ..
When the color tests keep revealing the presence of unreacted amino functions after recoupling, it is necessary to cap these to avoid the formation of deletion sequences. The capping will yield a truncated sequence (shortened pep- tide) but the truncated sequences differ generally considerably from the final peptide and can be readily separated.
Chemistry of Peptide Synthesis - CRC Press Book
The following protecting groups are compatible with HF cleavage: The following protecting groups are compatible with TFMSOTf cleavage: The following protecting groups are compatible with TMSOTf cleavage:
Coupling reactions in SPPS require the acylation reactions to be highly efficient to yield high-purity peptides.
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Better results will be obtained by repeating a coupling with fresh reagents (and changing coupling parameters if a low conversion was obtained) rather than by prolonging the reaction. Generally, coupling protocols may be changed in the course of a synthesis, especially when optimizing an SPPS.
Considering having a custom peptide synthesized
- the choice of the solvent. It will in part determine the swelling of the peptide-resin and influence the accessibility to the reactive sites; it will also have a direct effect on the kinetics of the coupling reaction.
- the steric hindrance. It is determined by the nature of the side chains R1 and R2 and of their protecting groups.
- the reactivity of the activated carboxylic acid.