This step is believed to be facilitated by unidentified proteins.
Design, Synthesis, and Antibody Binding Study.
The other approach is to introduce backbone protecting groups which will prevent the formation of hydrogen bonds. Such protection is made by the introduction of the Hmb group on the αnitrogen . It has been shown that the presence of a Hmb unit every 6-7 residues is sufficient to disrupt the peptide aggregation . The Hmb protected amino acid is introduced under the form of N,O-bis-Fmoc-N-(2hydroxy-4-methoxybenzyl) derivative, the O-Fmoc protection being cleaved during the following piperidine treatment. At the end of the synthesis the Hmb group is cleaved in the final TFA cleavage.
Fig. 8.16 Translation process of protein synthesis in prokaryotes.
Two approaches can be used to disrupt the aggregation; the first one consists in modifying the environment in which the synthesis is made and to introduce elements known to disrupt hydrogen bonds; among them we list:
Synthesis of Cyclo-PMMA via Click Chemistry Combined with ATRP.
To complete (+)DNA strand synthesis, an intramolecular transfer is required to give the (+)DNA strand access to the uncopied portion of the (-)DNA strand.
Synthesis of Lanthanide(III) Chelates by Using 'Click' Chemistry.
A special paragraph will be dedicated to the problems caused by peptide aggregation in the course of the synthesis. This phenomenon is a major cause of trouble as it is difficult to predict, is sequence dependent and no universal solution has been found up to now.
Synthesis of Ferrocene-containing Polyacetylenes by Click Chemistry.
A long-term oral anticoagulant therapy results in suppression of the synthesis of both vitamin K-dependent coagulation factors and Protein C, but the production of the coagulant and anticoagulant proteins is well-balanced.