Simonds Drawing Video:Summary of Protein Synthesis
T7 () and SP6 ()RNA polymerases are DNA dependent RNA polymerases that produce DNAtemplated RNA transcripts. T7 and SP6 exhibit high specificity for theirrespective promoters. Both T7 and SP6 can be used for the in vitrosynthesis of RNA for a wide variety of applications, includingtransfection, translation, structural studies and radioactive andnon-isotopic probe generation. E. coli Poly(A) Polymerase () and Poly(U) Polymerase () generate untemplated homoribopolymeric tails on the 3´-ends of RNA. Both E. coli Poly(A) Polymerase and Poly(U) Polymerase can be used for RNA tailing for reverse transcription or labeling.
This enzyme yields single stranded DNA on RNA template.
From RNA to Protein Synthesis - YouTube
The reverse transcriptase uses the free end and synthesizes a single stranded cDNA in the presence of dCTP, dGTP, dATP and dTTP, and results in mRNA-cDNA hybrid.
RNA Protein Synthesis - YouTube
mRNA peels off the DNA and moves out of the nucleus into the cytoplasm." title="Process of transcription"> Transcription takes place in the nucleus: Translation takes place on the ribosomes in the cytoplasm, or found on the rough Endoplasmic Reticulum (ER):